A β-galactosidase from Kluyveromyces lactis was found to specifically catalyze hydrolysis of the glycosyl ester linkage of stevioside to yield steviolbioside, a rare sweetener that also exists in Stevia rebaudiana leaves. In a packed bed reactor, a reaction coupling separation was realized and a production yield of steviolbioside reached 90% in 6 h. The hydrolysis product steviolbioside presented higher cytoxicity on human normal cells (hepatocytes cell L02 and intestinal epithelial cell T84) than stevioside did. Comparing to the typical chemotherapy agent, 5-fluorouracil (5-FU), steviolbioside presents much lower cytotoxicity on all assayed human normal cells; it presented notable inhibition on human hepatocarcinoma cell Hep3B, human breast cancer cell MDA-MB-231 and human pancreatic cancer cell BxPC-3. The remarkable inhibition on MDA-MB-231 cells makes steviolbioside a potential remedy for human breast cancer, when steviolbioside is served as a natural sweetener. #Stevia#kombucha#ikombucha_ro#ikombucha https://pubmed.ncbi.nlm.nih.gov/26593477/
Regarding nutritional benefits, the available studies, while numerous, do not provide proof that the consumption of artificial sweeteners as sugar substitutes is beneficial in terms of weight management, blood glucose regulation in diabetic subjects or the incidence of type 2 diabetes. Regarding nutritional risks (incidence of type 2 diabetes, habituation to sweetness in adults, cancers, etc.), it is not possible based on the available data to establish a link between the occurrence of these risks and the consumption of artificial sweeteners. However, some studies underline the need to improve knowledge of the links between intense sweeteners consumption and certain risks. #Stevia#kombucha#ikombucha_ro#ikombucha https://pubmed.ncbi.nlm.nih.gov/26430511/
The essential oil extracted by hydrodistillation from the flowering twigs of Stevia rebaudiana Bertoni (Asteraceae) was fractioned by chromatography. Forty-three constituents were characterized with the help of GC, GC-MS and other spectroscopic techniques. The essential oil was found to be a complex mixture of mono- and sesqui-terpenes. The cytotoxicity of the essential oil and its fractions was evaluated by sulforhodamine B (SRB) based assay against two cancer cell types viz. C-6 (rat glioma cells) and CHOK1 (Chinese hamster ovary cells). The essential oil and its fractions showed promising cytotoxicity against both cell lines. The highest activity (95.6+/-0.6%) was show by the essential oil on the C-6 cell line at a concentration of 400 microg/mL, which was comparable with that of the standard drug vinblastin. #Stevia#kombucha#ikombucha_ro#ikombucha https://pubmed.ncbi.nlm.nih.gov/25026731/
Stevia, a zero-calorie sugar substitute, is recognized as safe by the Food and Drug Administration (FDA) and the European Food Safety Authority (EFSA). In vitro and in vivo studies showed that stevia has antiglycemic action and antioxidant effects in adipose tissue and the vascular wall, reduces blood pressure levels and hepatic steatosis, stabilizes the atherosclerotic plaque, and ameliorates liver and kidney damage. The metabolism of steviol glycosides is dependent upon gut microbiota, which breaks down glycosides into steviol that can be absorbed by the host. In this review, we elucidated the effects of stevia's consumption on the host's gut microbiota. Due to the lack of randomized clinical trials in humans, we included in vitro using certain microbial strains and in vivo in laboratory animal studies. Results indicated that stevia consumption has a potential benefit on the microbiome's alpha diversity. Alterations in the colonic microenvironment may depend on the amount and frequency of stevia intake, as well as on the simultaneous consumption of other dietary components. The anti-inflammatory properties of stevioside were confirmed in vitro by decreasing TNF-α, IL-1β, IL-6 synthesis and inhibiting of NF-κB transcription factor, and in vivo by inhibiting NF-κB and MAPK in laboratory animals. #Stevia#kombucha#ikombucha_ro#ikombucha https://pubmed.ncbi.nlm.nih.gov/35456796/
Steviol glycosides (SGs) in Stevia (Stevia rebaudiana Bertoni) leaves are important due to their high sweetness and low calorific value. The yield of SGs is dependent on fertilization regimes, but the relationship between nitrogen (N) administration and SGs synthesis is still unclear. In this study, we investigate the effects of N rates on SGs production through hydroponic and plot experiments. The SGs yield was not significantly changed by N fertilization, but leaf SGs concentrations were significantly reduced due to the "dilution effect". Additionally, N addition decreased leaf carbon (C)/N ratio and soluble sugar concentration, accompanied with the inhibited phosphoenolpyruvate carboxylase and L-phenylalanine ammonia_lyase activities. A significant positive correlation between leaf SGs concentrations, C/N ratio and soluble sugar concentration was observed. Overall, we suggest that N-driven Stevia growth negatively affects SGs concentrations. The leaf C/N ratio and soluble sugar changes indicated the occurrence of metabolic reprogramming. #Stevia#kombucha#ikombucha_ro#ikombucha https://pubmed.ncbi.nlm.nih.gov/31195254/
Plant growth and secondary metabolism are commonly regulated by external cues such as light, temperature and water availability. In this study, the influences of low and high temperatures, dehydration, photoperiods, and different growing stages on the changes of steviol glycosides (SGs) contents and transcription levels of fifteen genes involved in SGs biosynthesis of Stevia rebaudiana Bertoni were examined using HPLC and RT-PCR. The observations showed that the transcript levels of all the fifteen genes were maximum under 25 °C treatment, and the transcription of SrDXS, SrDXR, SrMCT, SrCMK, SrMDS, SrHDS, SrHDR, SrIDI, SrGGDPS, SrCPPS1, SrUGT85C2 and SrUGT76G1 were restrained both in low temperature (15 °C) and high temperature (35 °C). Most genes in SGs biosynthesis pathway exhibited down-regulation in dehydration. To elucidate the effect of photoperiods, the plants were treated by different simulated photoperiods (8 L/16 D, 1 0L/14 D, 14 L/10 D and 16 L/8 D), but no significant transcription changes were observed. In the study of growing stages, there were evident changes of SGs contents, and the transcript levels of all the fifteen genes were minimal in fast growing period, and exhibited evident increase both in flower-bud appearing stage and flowering stage. The obtained results strongly suggest that the effect of environmental cues on steviol glycosides contents and transcription of corresponding biosynthetic genes in S. rebaudiana is significant. It is worth to study deeply. #Stevia#kombucha#ikombucha_ro#ikombucha https://pubmed.ncbi.nlm.nih.gov/25500454/
More than 60 naturally occurring steviol glycosides in the Stevia rebaudiana Bertoni plant share a similar molecular structure with an aglycone steviol backbone conjugated with β- and α-glycosidic bonds to different sugar moieties. These glycosides are naturally produced in different quantities within the stevia leaf. Certain minor glycosides with superior sensory attributes, such as Reb D and Reb M, are found less than 0.1% in traditional stevia leaves. New technologies can now produce better tasting steviol glycosides by using enzymatic conversion of stevioside and Reb A, which are abundant in stevia leaf. Several regulatory authorities recently evaluated steviol glycosides produced by enzymatic conversion of stevia leaf extract and approved them safe for human consumption. Steviol glycosides undergo microbial hydrolysis in the colon to generate steviol, which is absorbed and metabolized into steviol glucuronide, and excreted primarily via human's urine. Previous studies have shown the hydrolysis of highly purified individual steviol glycosides extracted from stevia leaf are converted to steviol in the presence of colonic microbiota of adults. Since colonic microbiota of children may be different from adults, this study investigates the metabolic fate in the colonic microbiota of adults and children of the minor steviol glycosides produced by extraction and enzymatic conversion of major steviol glycosides from stevia leaf. Several in vitro incubation tests were conducted in human fecal homogenates collected from adult and pediatric populations with steviol glycoside test samples comprised of a complex stevia leaf extract, a blend of minor glycosides isolated from stevia extract and two mixtures of steviol glycosides produced by enzymatic conversion of Reb A to larger molecules by attaching glucose units via β- or α-glycosidic bonds. Results from these studies clearly demonstrate steviol glycosides produced by extraction from stevia leaf, or enzymatic conversion of stevia leaf extract, share the same metabolic fate in the human gut microbiota from adults and children. Considering a common metabolite structure and a shared metabolic fate in all ages, safety data for individual steviol glycosides can be used to support safety of all steviol glycosides produced by extraction and enzymatic conversion of stevia leaf extract. #Stevia#kombucha#ikombucha_ro#ikombucha https://pubmed.ncbi.nlm.nih.gov/32745585/
Stevia rebaudiana Bertoni is a valuable plant whose products are increasingly used in medicine, pharmacy and the food industry. This necessitates the use of biotechnological approaches for its mass propagation. Establishing optimal conditions for in vitro cultivation is essential for obtaining high biomass and secondary metabolites production. A large number of articles considering the role of plant growth regulators and other additives in the culture medium in the growth and development of Stevia are available in the literature. However, there are no summarized data about the use of nanoparticles in Stevia tissue cultures. Therefore, this review also includes the research conducted so far on the effect of nanoparticles on Stevia micropropagation. Furthermore, the influence of different elicitors on secondary metabolite production and antioxidant activity of in vitro-cultivated Stevia plants have been discussed. By referring to the collected literature, we concluded that biotechnological approaches applied to S. rebaudiana cultivation might improve the agronomic traits of plants and steviol glycosides production. #Stevia#kombucha#ikombucha_ro#ikombucha https://pubmed.ncbi.nlm.nih.gov/36616282/
Recent research dives deep into the impact of autism on marital bonds. Over a 30-year span, parents of children with autism showcased a 36% divorce rate, with specific peak periods. Surprisingly, families of verbally adept autistic teenagers faced unique stressors, leading to higher divorce rates. The strength and resilience of these families remind us of the unique challenges they face.
Moving from Sydney to Melbourne can be an exciting adventure, but it can also be a daunting task filled with stress and anxiety. One of the crucial decisions you'll need to make is choosing the best “Removalists Sydney” to Melbourne. Whether you're relocating for work, family, or personal reasons, a professional removalist service can make your move smoother and less stressful. In this guide, we will explore the key factors to consider when selecting the best removalists in Sydney for your journey to Melbourne.
Reputation and Experience:
When searching for removalists in Sydney, it's essential to start by assessing their reputation and experience. Look for companies that have a solid track record of successful interstate moves. Reading customer reviews and testimonials can provide valuable insights into their service quality and reliability. Established companies with years of experience are more likely to handle your move with expertise and professionalism.
Services Offered:
Not all removalists offer the same range of services. Depending on your specific needs, you may require services such as packing, unpacking, storage, and vehicle transportation. It's essential to choose a removalist company that can tailor their services to meet your requirements. Whether you need a full-service package or only specific services, make sure the removalist can accommodate your needs.
Licensed and Insured:
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Competitive Pricing:
Moving can be costly, so it's important to get quotes from multiple removalists in Sydney to compare pricing. However, don't just opt for the cheapest option without considering other factors like reputation and services offered. Look for a balance between affordability and quality to ensure you receive value for your money.
Transparent Contracts:
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Customer Support:
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Conclusion:
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HVAC Electronically Commutated Motor Market Size to increase at a CAGR Of 7.5% during 2023-2028
The report discusses everything a marketer requires before investing in the global HVAC Electronically Commutated Motor Market Size 2023 during the forecast period 2023-2028. It provides detailed insight into current trends, market shares, market size, and sales value and volume.
The data used for this report is obtained from reliable industry sources, paid resources, and validated sources. This research works as a systematic guideline for marketers to make well-informed decisions. Key players in the global HVAC Electronically Commutated Motor Market are profiled with detailed insights into company profiles, product portfolio, geographical presence, statistical analysis, key developments, and growth strategies. Furthermore, the global HVAC Electronically Commutated Motor Market report provides a comprehensive analysis of the COVID-19 pandemic and how it has affected the market in the discussion. The study lays out how the pandemic affected the dynamics of the market and what are the future opportunities for market players.
The report discusses everything a marketer requires before investing in the global Latin America Power Distribution Component Market Size 2023 during the forecast period 2023-2029. It provides detailed insight into current trends, market shares, market size, and sales value and volume.
The data used for this report is obtained from reliable industry sources, paid resources, and validated sources. This research works as a systematic guideline for marketers to make well-informed decisions. Key players in the global Latin America Power Distribution Component Market are profiled with detailed insights into company profiles, product portfolios, geographical presence, statistical analysis, key developments, and growth strategies. Furthermore, the global Latin America Power Distribution Component Market report provides a comprehensive analysis of the COVID-19 pandemic and how it has affected the market in the discussion. The study lays out how the pandemic affected the dynamics of the market and what are the future opportunities for market players.
Detailed Overview
Market Dynamics
Detailed Market Segmentation
Historical, Current, and Projected Market Size in terms of volume and value
Market Trends and Developments
Competitive Landscape
Strategies of Key Players
Potential and Niche Segments
Key Questions Answered in Latin America Power Distribution Component Market Size:
What is the market size and CAGR of the global as well as the regional market?
What are the key players operating in the global Latin America Power Distribution Component Market?
What are the drivers, restraints, and growth opportunities of the market?
What are key technological trends in the market?
What are the various regions and sub-regions with leading contributions in the market?
On-demand Custom Research Report:
This report also offers specific consumer preferences and expectations to gain insights into changing needs and behavior of the consumer. We focus on more usefulness and actionable information specifically tailored to suit your business needs.
The cycle of disruption and transformation is imminent for businesses looking to carve out a niche in highly competitive environments. While a few companies are focusing on transformational growth and addressing key challenges of disruptions, several others are simply unaware. At Fortune Business Insights we offer end-to-end solutions beyond flagship research technologies to help senior leaders across enterprises achieve their mission-critical goals. We deliver recommendations enabling growth to help build the organizations of tomorrow.
Why we have to use InDriver clone app?
In the fast-paced world of today, the way we commute has evolved significantly. Gone are the days when hailing a taxi or waiting for a ride involved extended wait times and uncertain fare rates. Thanks to innovative ride-sharing platforms like InDriver, the ride-hailing experience has been revolutionised. In this article, we will explore the reasons why using an InDriver clone app is a smart choice for both riders and entrepreneurs looking to enter the transportation industry.
What is InDriver?
InDriver is a ride-hailing platform that has taken the world by storm since its inception. It distinguishes itself from traditional ride-hailing apps by allowing passengers to set their fares and drivers to accept or negotiate those fares. This unique pricing model empowers riders by giving them more control over their transportation costs while ensuring that drivers are fairly compensated for their services. The InDriver platform has gained popularity in various regions due to its user-centric approach and fair pricing mechanism.
Why Use an InDriver Clone App?
Empowerment for Riders
One of the primary reasons to opt for an InDriver clone app is the empowerment it offers to riders. Traditional ride-hailing apps dictate the fares, leaving passengers at the mercy of surges and fluctuating pricing algorithms. In contrast, InDriver clone apps allow passengers to set their own fares based on their budget and preferences. This transparency and control over pricing give riders a sense of empowerment that is hard to find in other ride-hailing services.
Fair Earnings for Drivers
For drivers, an InDriver clone app presents an opportunity to earn a fair wage for their services. The ability to negotiate fares means that drivers can accept rides that align with their income goals. This results in a more satisfied driver pool, leading to better service quality and ultimately benefiting riders as well.
Enhanced Competition
Competition is a driving force behind innovation and improved services. By using an InDriver clone app, entrepreneurs can enter the ride-hailing market with a unique proposition. The competition will encourage existing players to adapt and offer more flexible pricing models, ultimately improving the overall ride-hailing experience for everyone.
Greater Transparency
Transparency is a crucial aspect of any successful business model, and InDriver clone apps excel in this regard. Both riders and drivers have full visibility into the pricing negotiation process. This transparency builds trust among users and enhances the overall reputation of the service.
Versatility and Customization
InDriver clone apps are not limited to traditional taxi services. They can be customised to offer various transportation options, including carpooling, luxury rides, and even delivery services. This versatility allows entrepreneurs to tap into multiple segments of the transportation market, diversifying revenue streams.
Regional Expansion
InDriver clone apps can be tailored to suit the specific needs and preferences of different regions. This flexibility enables entrepreneurs to expand their services to new markets with ease, catering to the unique demands of each location.
Conclusion
In summary, the use of an InDriver clone app offers a multitude of benefits for both riders and entrepreneurs. This innovative approach to ride-hailing empowers passengers, ensures fair compensation for drivers, and fosters healthy competition in the transportation industry. The transparency, versatility, and potential for regional expansion make InDriver clone apps a smart choice for those looking to disrupt the ride-hailing market. As the transportation landscape continues to evolve, InDriver and its clones represent a promising path forward for the industry, promising a brighter future for all stakeholders involved.
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In today's fast-paced world, the convenience of ride-hailing services has become an integral part of our daily lives. One name that has gained prominence in this arena is InDriver. However, have you ever wondered why people are increasingly turning to InDriver clone websites? In this article, we will explore the reasons behind the growing popularity of InDriver clone websites and how they are revolutionising the ride-hailing industry.
What is InDriver?
Before we delve into the world of InDriver clone websites, let's briefly understand what it is. This is a global ride-hailing platform that allows passengers to negotiate the fare directly with drivers, offering a unique and transparent pricing system. With its presence in numerous countries, it has disrupted traditional ride-hailing by putting more control into the hands of passengers.
The Appeal of InDriver
InDriver's success can be attributed to several key factors. First and foremost is its transparent pricing model. Passengers can input their desired fare, and nearby drivers can accept or counteroffer. This approach empowers passengers by giving them a say in the price they are willing to pay for a ride, eliminating any potential surprises at the end of the journey.
Moreover, InDriver offers a wide range of vehicle options, from budget-friendly rides to more premium experiences. This flexibility caters to diverse customer preferences and budgets, making it a go-to choice for people with varying transportation needs.
In addition to these features, it also focuses on safety and security, with thorough driver background checks and in-app communication tools that allow passengers to share their trip details with loved ones. All these aspects combined make this a popular choice among ride-hailing users.
The Emergence of InDriver Clone Websites
Now, let's talk about why InDriver clone websites have gained traction. InDriver clone websites replicate the functionality and user experience of InDriver, offering a similar platform with a few unique advantages.
Geographic Expansion: It might not be available in all regions or countries, leaving a gap in the market for ride-hailing services. InDriver clone websites bridge this gap by offering a similar experience to users in areas where ,It has not yet expanded.
Customization: InDriver clone websites allow entrepreneurs and businesses to customise the platform to suit their specific needs. They can adapt the service to cater to niche markets or unique features, providing a tailored experience to their customers.
Competitive Edge: By utilising InDriver's proven model, clone websites can enter the ride-hailing market with a competitive advantage. Users who are already familiar with it may
find these clone services attractive due to their similarity.
Cost-Effective Solution: Developing a ride-hailing app from scratch can be a costly and time-consuming process. In contrast, InDriver clone websites offer a cost-effective solution, saving both time and money for entrepreneurs looking to enter the ride-hailing industry.
Conclusion
InDriver and its clone websites have transformed the way we approach ride-hailing services. While it continues to be a popular choice for passengers seeking transparent pricing and a wide range of vehicle options, InDriver clone websites have emerged to cater to untapped markets and provide customised solutions.
Whether you're in an area where it operates or not, the presence of this clone website expands the reach of this innovative ride-hailing concept. These platforms offer a cost-effective, customizable, and competitive alternative, giving entrepreneurs the opportunity to enter the ride-hailing market and provide passengers with the convenience and transparency they seek.
In conclusion, the use of these clone websites is a testament to the ever-evolving nature of the ride-hailing industry. As technology continues to advance, we can expect further innovations and adaptations in this sector, ultimately benefiting passengers with more choices and enhanced user experiences.
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When you're ready to buy Tramadol, make sure you check out a few different websites to find the best deal. You may be able to find a better price by shopping around. In addition, you should check to see if the website is offering free shipping and quick overnight delivery in the USA. This will help you get your product quickly and without any extra cost.
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